Figure 3. Synaptic mAb7a-specific gephyrin clusters are reduced upon CDK5 knockdown whereas VIAAT puncta were not reduced in perisomatic dendritic segments.

Hippocampal neurons (div14) were immunolabeled with anti-GFP antibodies to detect infected neurons (upper panel), with phosphospecific anti-gephyrin mAb7a antibody (middle panel), and with anti-VIAAT antibody (lower panel). (A) Non-infected cells; (A') CDK5-kd2 knockdown; (A'') control shRNA (CDK5-mismatch). Neurons were infected with the indicated viruses at div6. The scale bar represents 15 µm. (B) Quantification of the number of VIAAT puncta, mAb7a puncta and overlapping mAb7a/VIAAT puncta (synaptic mAb7a clusters). Mean ± SE. Each value was calculated from analysis of 15 cells from n = 3 independent cultures, ANOVA with post-hoc test: *** P< 0.001, * P<0.05.