Skip to main content
. 2014 Aug 5;9(8):e104107. doi: 10.1371/journal.pone.0104107

Figure 2. In vivo recruitment of neutrophils into the peritoneal cavity.

Figure 2

Mice received an i.p. dose of 1-2(7–98), or a mixture of both COAM (1 mg) and mouse GCP-2(7–98) (100 ng). After 1 h (A) or 4 h post-treatment (B), peritoneal lavage fluids were collected and the percentages and absolute numbers of neutrophils, recognized as CD11b and Ly6G double positive cells, determined by FACS analysis, are shown. The net numbers of CD11b+ Ly6G+ cells were determined by multiplying the percentages of CD11b+ Ly6G+ cells with total peritoneal leukocyte counts. Histograms and dots represent group medians and spreading of individual data points from each mouse, respectively. *P<0.05, **P<0.01, ***P<0.001, as determined by Kruskal-Wallis test.