Figure 2.
Preparation of rhGH microspheres by an atomizer.
Notes: Reprinted from Pharmaceutical Research, 14(6), 1997, pages 730–735, The stabilization and encapsulation of human growth hormone into biodegradable microspheres, Johnson OL, Jaworowicz W, Cleland JL, et al, Figure 1.16 With kind permission from Springer Science and Business Media. Copyright © 1997, Plenum Publishing Corporation. The rhGH:Zn complex was suspended in dichloromethane containing PLGA polymer. The suspension was atomized by an ultrasonic nozzle and frozen by liquid nitrogen. After the evaporation of liquid nitrogen, the ethanol melted and extracted dichloromethane from the microspheres, which were then filtered and dried.
Abbreviations: PLGA, poly(lactic-co-glycolic acid); rhGH, recombinant human growth hormone.