Skip to main content
. 2014 Aug 5;9(8):e104062. doi: 10.1371/journal.pone.0104062

Figure 4. MND-tdTomato expression following injection with pulled glass pipette.

Figure 4

Confocal images of sagittal cerebellar slices from wild type mice injected with MND-tdTomato lentivirus. Injections were performed using pulled glass pipettes and a picospritzer (see methods) to determine if injection technique affected cellular transduction pattern. Dotted lines in A and B represent the border between the Purkinje layer and granule layer. In C, dotted lines are drawn to separate the Purkinje layer from the molecular layer and granule layer. A. Widespread tdTomato expression in cells in the granule layer and processes in the molecular layer. B. A few small tdTomato expressing cells are visible in the Purkinje layer, but the majority of the tdTomato expressing cell bodies are located in the white matter (wm). C. High magnification view of tdTomato expressing cells with somata in the Purkinje layer show that their processes are relatively straight and unbranched, characteristic of Bergmann glia. m, molecular layer; p, Purkinje layer; g, granule layer. Scale bars: 100 µm (A, B); 25 µm (C).