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. 2014 Aug 5;9(8):e104062. doi: 10.1371/journal.pone.0104062

Figure 7. AAV1 delivery of a dual promoter construct reveals that the CAG promoter produces better Purkinje neuron expression than the PGK promoter.

Figure 7

A. Schematic representation of the dual promoter AAV transfer construct which contains the CAG promoter followed by FGF14A and the PGK promoter followed by GFP. CAG, chicken β-actin promoter containing the CMV enhancer; in, SV40 intron; PGK, human phosphoglycerate kinase promoter; pA, polyadenylation site; ITR, inverted terminal repeat. B. Confocal images of sagittal sections from an Fgf14−/− mouse injected with the AAV-CAG-dual promoter virus and immunostained for FGF14 (red) and AnkyrinG (blue). Viral delivered FGF14 is properly localized at the Purkinje neuron AIS where it colocalizes with AnkyrinG. A lower level of FGF14 expression is visible on the soma membrane. Viral delivered GFP is expressed in small cells in the Purkinje layer that extend radial processes into the molecular layer (a pattern that is consistent with Bergmann glia) but is clearly absent from Purkinje neurons. Scale bars: 25 µm (B, top); 5 µm (B, bottom).