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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Eur J Neurosci. 2014 Mar 11;39(9):1439–1454. doi: 10.1111/ejn.12516

Figure 2.

Figure 2

Confocal images of NK1R neurons after intrathecal BDNF and NMDA. Rats received (A) intrathecal injections of saline or (B–D) 10 nmol NMDA and D-Ser, and were fixed 10 min later for NK1R immunohistochemistry. Images were taken from the L5 spinal segment. (A) Injection of vehicle (saline) produced no NK1R internalization. (B) Injection of NMDA and D-Ser produced no NK1R internalization. (C) BDNF (3 μg) was injected intrathecally 60 min before NMDA, resulting in NK1R internalization in more than half of the neurons. (D) The SFK inhibitor PP2 (10 nmol) was coinjected with BDNF (3 μg) 60 min before NMDA, resulting in no NK1R internalization. Main panels: images taken with a 10× objective; voxel size of 830 × 830 × 5983 nm and three confocal planes. Insets: images taken with a 63× objective of the lamina I neurons indicated by the frames in the main panels; voxel size of 132 × 132 × 383 nm and three confocal planes. Adjustments in the gamma of the images in the insets were made to ensure that the staining of neurons was of similar intensity; the uncorrected intensity can be seen in the main panel images. Neurons with NK1R internalization are indicated with ‘*’ and neurons without internalization by ‘o’, with the symbol placed over the nucleus. Scale bars, 100 μm (main panels), 10 μm (insets).