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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Eur J Neurosci. 2014 Mar 11;39(9):1439–1454. doi: 10.1111/ejn.12516

Figure 5.

Figure 5

BDNF increased Tyr phosphorylation of the NR2B subunit. (A) Western blots performed on extracts of DRG neurons cultured 2–3 days in the presence of ketamine and incubated 1 h with 20 ng/ml BDNF (BDNF) or without (control; CON). The blots were probed with antibodies to (pTry1472)-NR2B (p-NR2B), NR2B and β-actin. NR2B and p-NR2B had an apparent molecular weight of 190 kDa. (B) The intensity of the bands corresponding to the p-NR2B and NR2B antibodies were normalized to actin and expressed as the ratio of BDNF versus control. *P = 0.0185 compared to NR2B, t-test, n = 6. Expressed as the ratio of p-NR2B to NR2B for each experimental pair, BDNF increased NR2B-phosphorylation an average of 2.2 ± 0.4-fold.