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. 2014 Apr 23;40(3):2502–2512. doi: 10.1111/ejn.12603

Figure 6.

Figure 6

Toll-like receptors have broad expression in the retina. (A) Micrograph of the gel-separated PCR products from the RT-PCR test showing specific bands corresponding to mRNA for Tlr1,Tlr2,Tlr3,Tlr4,Tlr7 and Tlr9 detected in the retinas of sham-operated (sham) mice and in the ischemic retinas. (B) Differential expression of TLRs in sham-operated and ischemic retinas 6 h after reperfusion was tested by quantitative RT-PCR. For each gene, results are expressed as a percentage of the corresponding value for Tlr1 expression in the sham-operated retina ± SE of the mean (**P < 0.01, *P < 0.05, n = 6). (C) Micrograph of the gel-separated PCR products from the qRT-PCR test showing specific bands corresponding to mRNA for Tlr3, Thy1 (RGC marker), Gfap (astroglial marker) and Cd11b (microglial marker) detected in the total RNA samples isolated from 500 and 100 microglial cells, astrocytes, and RGCs. RGCs were purified with a two-step immunopanning protocol from 12-day-old and 1-month-old mice. (D) Micrograph of the gel-separated qRT-PCR products for the members of the toll-like receptor family obtained by using total RNA isolated from control and OGD-treated primary RGC cultures. (E) Relative enrichment of Tlr2, Tlr3 and Tlr4 transcripts detected by qRT-PCR in control and OGD-treated RGCs (**P < 0.01, *P < 0.05, n = 6).