a-c, Cell proliferation rates (top panels) and colony formation in soft agar assays (bottom panels) of murine LAC cell line LKR10 (a), human LAC cell line A549 (b), or human PDAC cell line CFPac1 (c) with or without SMYD3 depletion by stable shRNA (respective immunoblot in middle panels). d-f, SMYD3 depletion in CFPac1 attenuates tumor growth in mouse xenografts. d, Macroscopic picture of xenografts from control and SMYD3 knock-down tumors at the end of the experiment. Scale bar, 1 cm. e, Volume analysis shows that shSMYD3 significantly inhibits the expansion of pancreatic tumors (n=6 for each group). f, HE of the tumors and IHC confirmation of SMYD3 expression and knock-down. All scale bars, 50 μm. *: p-value<0.05; **: p-value<0.01; ***: p-value<0.001 (two-tailed unpaired Student's t-test). Data are represented as mean +/− SEM.