Fig 6.

Comparative BONCAT analyses of a methanotrophic enrichment culture in the absence and presence of methane. A. Click chemistry-mediated detection of AHA incorporation (green) reveals that a gammaproteobacterium (identified by FISH probe MetI-444; red) is highly active in the presence but not in the absence of methane. B. To test whether AHA-labelled WF1 cells (examples are pointed out by arrows) would be detectable in a complex samples, an aliquot of the culture was spiked into methane seep sediment and analyzed via BONCAT. Exposure settings for recording of BONCAT signals were identical for each image set (i.e. A, B). For images taking at different settings and controls, see Supporting Information Fig. S5. Scale bars equal 10 μm and apply to all images of the respective set.