Fig. 1.

Obese skeletal muscle has elevated REDD1 (regulated in development and DNA damage responses 1) protein expression. Equal protein from fasted lean and ob/ob male mice plantar flexor complex muscle homogenates were analyzed by Western blot analysis for REDD1, hypoxia-inducible factor (HIF)-1α, phospho-4E-BP1 T37/46, and actin, then normalized to actin. Representative Western blots are shown. Means marked with an * are significantly different, P < 0.05 vs. lean (n = 10/group). Inset: C2C12 myotube lysates analyzed for REDD1 and HIF1α protein expression following either 3 h of normoxia or hypoxia.