Fig. 3.

The CR variant exhibits a weaker dominant-negative effect on the endogenous TRESK currents in trigeminal ganglion (TG) neurons than MT TRESK subunits. A: voltage protocol used to minimize transient voltage-gated K⁺, Na⁺, and Ca²⁺ currents and the representative current traces from TG neurons expressing mCherry protein, mCherry-CR TRESK variant, and mCherry-MT TRESK subunits before and after the application of 30 μM lamotrigine, respectively. B: percentage of outward current (measured at the end of the depolarizing step) inhibited by lamotrigine (n = 12–18 neurons in each group; one-way ANOVA with post hoc Bonferroni test; ***P < 0.001, compared with the mCherry group; #P < 0.05).