Fig. 5.
Overexpression of the TRESK CR variant does not alter the excitability of small isolectin B4 (IB4)-negative TG neurons. A: representative traces of action potentials (APs) generated by incremental depolarizing current injections in transfected, small IB4-negative TG neurons. The values of resting membrane potential (Vrest) and the injected current are indicated. B: mean rheobase of the small IB4-negative TG neurons expressing the mCherry proteins, mCherry-CR, and mCherry-MT TRESK subunits, respectively (n = 9–18 neurons in each group; *P < 0.05, ***P < 0.001, one-way ANOVA with post hoc Bonferroni test). C: input-output plots of the transfected, small IB4-negative TG neurons (same as in B). The spike frequency was measured by injection of 1-s depolarizing current in 25-pA incremental steps in each neuron [two-way repeated-measures (RM) ANOVA and post hoc t-test with Bonferroni correction, *P < 0.05, **P < 0.01, compared with the corresponding mCherry groups; ##P < 0.01, compared with the corresponding mCherry-CR groups].