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. 2014 May 21;112(4):834–844. doi: 10.1152/jn.00228.2014

Fig. 6.

Fig. 6.

EFP responses are Mauthner cell dependent. A: selective apoptosis in neurons expressing epNTR during metronidazole treatment. In a y264;UAS:epNTR-RFP larva with stochastic epNTR-RFP expression in the left Mauthner cell (arrow, top), PhiPhiLux G1D2 fluorescence is observed only in the same cell (arrow, bottom). A, anterior. B: acoustic LLC responsiveness and kinematics in y264;UAS:epNTR-RFP ablated larvae (gray) and nonablated sibling controls (black) (n = 4 plates of 5 fish for each group). C: EFP responsiveness in y264;UAS:epNTR-RFP ablated larvae (gray, n = 15 fish) and nonablated sibling controls (black, n = 17 fish). *t-Test, P < 0.01. D: immunolabeling of GFP in J1229 larvae after laser ablation of the left (top) or both (bottom) Mauthner cells. E and F: EFP responsiveness (E) and response direction (F; % of responses directed rightward) after unilateral or bilateral Mauthner cell laser ablations. Remaining Mauthner cell(s) are depicted. n is indicated in parentheses. *t-Test, P < 0.01.