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. 2014 Jul 21;15:90. doi: 10.1186/1471-2202-15-90

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© Rockenstein et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Characterization of Tau/GSK3β bigenic mice. (A) Representative immunoblot image for total GSK3β (t-GSK3β), phospho-GSK3β (p-GSK3β)(Y216), total Tau (t-Tau) and phospho-Tau (p-Tau) levels in the hippocampus of the Tau/GSK3β bigenic mice compared to Tau tg, GSK3β tg and non-tg mice. Actin was used as a loading control. (B) Analysis of levels of t-GSK3β and p-GSK3β showing 3 fold higher levels in GSK3β bigenic mice. (C) Analysis of levels of t-Tau and p-Tau (AT8) showing higher levels of p-Tau in bigenic mice compared to Tau tg mice. (D, E) Immunohistochemical and image analysis of pGSK3β immunoreactivity in the dentate gyrus of the hippocampus (inset) in the non-tg, single tg and Tau/GSK3β bigenic mice. (F, G) Immunohistochemical and image analysis of p-Tau (AT8) immunoreactivity in the dentate gyrus of the hippocampus (inset) in the non-tg, single tg and Tau/GSK3β bigenic mice The boxes indicate regions shown at higher magnification in the lower panels. Scale bar in upper panel = 250 μm, in lower panels = 30 μm. Error bars represent mean ± SEM (n = 6 per group, 6 m/o). *Indicates a significant difference (p < 0.05) between non-tg and single or bigenic mice analyzed by one-way ANOVA and Dunnett’s post hoc test. # Indicates a significant difference (p < 0.05) between Tau tg and bigenic mice analyzed by one-way ANOVA and Tukey-Krammer post hoc test.