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. 2014 Jul 29;14:547. doi: 10.1186/1471-2407-14-547

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© Yamauchi et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Ara-G cytotoxicity against mitochondrial DNA-depleted CEM (ρ ⁰ CEM) cells. (a) Determination of cytochrome c oxidase activity in ρ⁰CEM cells. The activity was completely suppressed in mitochondrial DNA-depleted variant cell line ρ⁰CEM as compared with CEM cells. (b, c) CEM cells and ρ⁰CEM cells were treated with 10 μM (b) or 100 μM (c) ara-G for 48 h. Sub-G1 induction was calculated by using flow cytometry. The values shown are the means ± SD of at least three independent experiments. The difference in the values between CEM cells and ρ⁰CEM cells was not significant for either concentration (P = 0.28 for 10 μM ara-G (b), P = 0.40 for 100 μM ara-G (c), unpaired T test).