The temporal precision of spikes firing in the ACC of mice in vitro. Aa, The traces showed 15 responses under the steady-state currents stimulations. Ab, The raster plot showed the AP firing recorded in Aa. B, The jitter and relative time of APs presented in A was fitted by a liner function; the slope indicated the change rate of five total APs. C, Represented data showed that the jitter of APs negatively correlated with the amplitude of test currents. Black, blue, and green indicats the slope of jitter change under low, medium, and higher intensity, respectively. Da–Dc, Representative examples of firing patterns of intermediate (IM) and regular spiking (RS) neurons respectively. E, Summarized data showed that no difference was detected on the total five APs. F, The summarized data represent the slope of jitter change of three types of pyramidal neurons. No difference was detected among groups.