Figure 6.

Glutamatergic instead of GABAergic synaptic transmission was involved in the change of temporal precision of AP firing induced by CFA injection. A, The application of mixture with CNQX (25 μm), AP5 (50 μm), and picrotoxin (100 μm) decreased the jitter of AP firing in CFA-injected mice, but not the control mice. B, The mixture application abolished the difference of jitter elicited by simulated EPSCs; * p < 0.05. C, Blocking of the synaptic transmission eliminated the change of jitter induced by nerve injury. D, Summarized data showed the functions of currents intensity and number of APs of neurons in the ACC from control and CFA-injected mice. E, No difference was detected on the rheobase and test currents, which was used to examine the jitter between control and CFA group. F, Blocking glutamatergic synaptic transmission by CNQX (20 μm) and AP5 (50 μm) in bath solution decreased the slope of jitter in CFA-injected mice, therefore abolished the difference between control (black) and CFA group (red). G, Blocking glutamatergic synaptic transmission by CNQX (25 μm) and AP5 (50 μm) in bath solution abolished the difference of jitter between control and CFA group. H, Bath application of picrotoxin (100 μm) did not abolish the difference of slope of jitter between control and CFA group (t test, p < 0.01). I, Bath application of picrotoxin (100 μm) did not abolish the difference of slope of jitter between control and CFA group (two-way ANOVA, p < 0.01).