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. 2014 Aug 5;8:66. doi: 10.3389/fnana.2014.00066

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Copyright © 2014 Schuster, Belur, Riedl, Schnell, Podetz-Pedersen, Kitto, McIvor, Vulchanova and Fairbanks.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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GFP expression in the choroid plexus. (A) GFP-ir in choroid plexus of the 4th ventricle. (B) DAPI staining in the same section showing cellular nuclei in the choroid plexus (center), cerebellum (top), and medulla (bottom left and right). (C) Overlay of images in (A) and (B) showing a substantial portion of the choroid plexus cells to be transduced. (D) Graph illustrating that transduction of choroid plexus cells is significantly higher in the 4th ventricle compared to the third and lateral ventricles. ^*Indicates significance as determined by one-way ANOVA followed by Bonferroni post-hoc analysis, (p < 0.001, n = 4). Across all animals and ventricles 35,071 nuclei were counted, 3708 of which were identified as GFP-positive.