Table 1.

Relative abundance of GFP-ir in brain after intrathecal AAV5-GFP delivery with an intravenous mannitol pretreatment (evaluated in 8 animals from two separate experiments; each structure was observed in at least 3 different sections).

Brain area	Neuronal somata	Neuronal fibers	Non-neuronal cells
Gracile/Cuneate n.	−	+++	−
Spinal Trigeminal n. and tract	+	++	−
NTS, Solitary tract, and AP	++	++	−
Vestibular/Cochlear n.	+	+	−
Facial n.	+	+	−
Cerebellum	++	++	−
4th Ventricle Choroid Plexus	−	−	++++
Pons	++	++	−
Inferior Colliculus	+	+	−
Superior Colliculus	+	+	−
Cerebral Peduncles	−	−	++
Medial Geniculate n.	++	++	−
LGN and Posterior Thalamic n.	+	++	−
Hypothalamus	+	+	−
3rd Ventricle Choroid Plexus	−	−	+++
Subiculum	+++	++	++
Entorhinal Ctx.	+++	++	++
Caudal visual & Retrosplenial Ctx.	++	++	++
Rostral visual & Retrosplenial Ctx.	+	+	+
Rostral−Ventral Hippocampal Form.	+++	++	+
Rostral−Dorsal Hippocampal Form.	−	+++	−
Amygdala	++	+	−
Lateral Ventricles Choroid Plexus	−	−	+++
Cingulate, Motor & Sensory Ctx.	+	+	+
Olfactory bulb	++	++	++

Definitions of relative abundance: −, no GFP labeling observed; +, isolated cells; ++, multiple labeled cells observed in close proximity to one another; +++, GFP-ir at least as prevalent as ++ throughout the structure, with some areas showing markedly increased GFP-ir; ++++ = a substantial fraction of cells throughout the entire structure showed GFP-ir. Abbreviations: n, nucleus; NTS, nucleus of the solitary tract; AP, area postrema; LGN, lateral geniculate nucleus; Ctx, cortex.