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. 2014 Aug 6;4:5973. doi: 10.1038/srep05973

Figure 3. A portion of G51R rhodopsin is transported to the lysosomes in the absence of Rer1p.

Figure 3

(A) Translocation of G51R mutant rhodopsin to lysosomes in Rer1 knockdown cells. HeLa cells expressing each FLAG-rhodopsin-GFP construct were transfected with control siRNA or Rer1 #2 siRNA. Cells were immunostained with an anti-LAMP1 antibody. The signals of FLAG-rhodopsin-GFP (green) and LAMP-1 (red) are shown in the merged images. Bar: 10 μm. (B) The graph shows the average number of rhodopsin-positive puncta per cell. Thirty cells were counted in each experiment. Three independent experiments were examined. Error bars represent the SE. * indicates p < 0.05.