Figure 4. Representative traces for the real-time measurement of intracellular Ca²⁺ in SH-SY5Y cells transfected with TRPM8 FK 1045,1046 AG mutant.

(a–e) Pre-treatment with PP2 caused a dose-dependent inhibition of agonist -induced elevation in intracellular Ca²⁺ (150 nM WS 12). PP3 did not inhibit responses to agonist, (e) The IC₅₀ for inhibition of TRPM8 response was approximately 24 μM PP2, (f) Dose–response experiments were repeated on more than three separate occasions.