Figure 1. heNOS and the deletion constructs.

(A) The relative positions of phosphorylation sites in heNOS. Blocks indicate the binding sites for haeme, H₄B, CaM, FMN, FAD and NADPH in eNOS. AIE and CT refer to the AIE and the C-terminal tail, respectively. The myristoylation site (Myr.) and palmitoylation sites (Palm.) are noted. Potential phosphorylation sites Ser¹¹⁴, Thr⁴⁹⁵, Ser⁶¹⁵, Ser⁶³³ and Ser¹¹⁷⁷ are indicated. (B) Constructs for the eNOS deletion mutants. The sequences of the AIE and the C-terminal tail are shown above and below, respectively. The Δ594 is a deletion of the AIE region encompassing residues Ser⁵⁹⁴–Glu⁶⁰⁴ (shown in blue); Δ605 is a deletion of the AIE region encompassing residues Gln⁶⁰⁵–Arg⁶¹² (shown in red); Δ626 is a deletion of the AIE region encompassing residues Trp⁶²⁶–Ser⁶³⁴ (shown in green); Δ14 is a deletion of 14 amino acid residues at the C-terminal tail encompassing residues Leu¹¹⁶⁴–Ser¹¹⁷⁷ (shown in violet).