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. 2014 Aug 6;34(4):e00129. doi: 10.1042/BSR20140079

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© 2014 The Author(s) This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

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Stable HEK-293 cell lines with (A) myr⁻eNOS-EGFP or (B) WTeNOS–EGFP were visualized in live cells by fluorescence microscopy. (C) cell lysates from HEK-293 cells transfected with empty vector (pCDNA3), Myr⁻eNOS or WTeNOS were immunoblotted for changes in phosphorylation state of Thr⁴⁹⁵, Ser⁶¹⁵, Ser⁶³³ and Ser¹¹⁷⁷. The expression of eNOS and β-actin was used as internal loading control. Western blots shown are the representatives of at least three independent studies.