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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Methods Mol Biol. 2014;1098:59–69. doi: 10.1007/978-1-62703-718-1_5

Fig. 2.

Fig. 2

Cell-based GLuc complementation assay for inhibition of CXCL12-CGLuc binding to NGLuc-CXCR4. Equal numbers (1 × 10 4 cells each) of MDA-MB-231 cells secreting CXCL12-CGLuc (231-CXC12-CGLuc) or expressing NGLuc-CXCR4 (231-NGLuc-CXCR4) were cocultured in black wall 96 plates. Cells were incubated with vehicle control (row A) or increasing concentrations of the CXCL12-CXCR4 inhibitor AMD3100, which decreased bioluminescence from GLuc complementation. Grid overlay is used for ROI analysis. Scale bar shows range of photon fl ux values by pseudocolor display with red being highest and blue lowest numbers