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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Methods Mol Biol. 2014;1098:59–69. doi: 10.1007/978-1-62703-718-1_5

Fig. 3.

Fig. 3

Imaging ligand–receptor binding in living mice. Mice were implanted with equal numbers of 231-CXCL12-CGLuc and 231-NGLuc-CXCR4 cells as ortho-topic mammary tumor xenografts in NSG mice. Imaging began 20 s after tail vein injection of coelenterazine, using 2 min acquisition and large binning. Circles and values show photon fl ux measurements for ROIs around each tumor. Scale bar shows range of values depicted by pseudocolor display