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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: J Pediatr Surg. 2014 May 16;49(8):1286–1291. doi: 10.1016/j.jpedsurg.2014.04.001

Figure 2.

Figure 2

S1P2 antagonist JTE-013 inhibited S1P-induced CCL2 expression in NB cells. (A and B) Serum-starved SK-N-AS cells were pretreated with JTE-013 (1μM) for 0.5 h and incubated with S1P (1μM) for 2 h (A) or 4 h (B) followed by quantitative real-time PCR (A) and CCL2 ELISA (B). (C and D) Serum-starved and JTE-013-pretreated SK-N-BE(2) cells were incubated with different concentrations of S1P for 2 h (C) or 4 h (D) followed by quantitative real-time PCR (C) and CCL2 ELISA (D). *, P< 0.05, **, P< 0.01 versus non S1P-treated controls. #, P < 0.01, ##, P < 0.01 as indicated.