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Overexpression of S1P2 increased CCL2 secretion in NB cells. SK-N-AS cells (A) or SK-N-BE(2) cells (B) were infected with S1P2 or GFP adenovirus with MOI 100, serum starved and stimulated with 1μM (A) or 100nM (B) of S1P in 1 ml SF media for 4 h followed by CCL2 ELISA. *, P< 0.05, **, P< 0.01 versus non S1P-treated controls. ##, P < 0.01 as indicated.
