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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: DNA Repair (Amst). 2014 Jun 13;21:12–23. doi: 10.1016/j.dnarep.2014.05.005

Fig. 3. RPA heterotrimeric phosphorylated isoforms before and after DNA damage in G2.

Fig. 3

Isoelectric focusing of heterotrimeric RPA was performed using a pH 5–6 gradient which was then developed with the RPA1-CT antibody. (A) Control (blue) vs. DNA damaged (pink) lysates. (B) Control (blue) vs. control phosphatase-treated (grey) lysates (C) DNA damaged (pink) vs. DNA damaged plus phosphatase-treated (grey) lysates. The pI values assigned to peaks by Compass software are shown. The data were collected in triplicate with undiluted RPA1-CT antibody and on different days and were reproducible.