Figure 6.

ASPP2 overexpression induces DRAM-mediated autophagic apoptosis. (a and b) Hep1-6 and Huh7 cells were transfected with the ASPP2 plasmid (ASPP2 Pl) for 24 h with or without co-transfection with p73 siRNA (p73 si). Real-time PCR and immunoblot assays were used to detect the mRNA (a) and protein (b, left panel) level of DRAM in Huh7 and Hep1-6 cells. Immunoblot assay was used to detect the effect of p73 si on DRAM, LC3 I/II, CHOP and PARP levels in Huh7 and Hep1-6 cells (b, right panel). (c) Immunoblot assay was used to detect the effects of DRAM knockdown via siRNA (DRAM si) on CHOP expression and the levels of autophagy and apoptosis in Hep1-6 and Huh7 cells with (left panel) or without (right panel) transfection with ASPP2 Pl. (d) M30 assay was used to detect the effects of DRAM knockdown on autophagy and apoptosis. (a and d) Data are shown as the mean±S.E.M. of three independent experiments