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. 2014 Jul 17;5(7):e1323. doi: 10.1038/cddis.2014.276

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Copyright © 2014 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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CHOP does not affect the expression of DRAM. (a–d) Hep1-6 and Huh7 cells were transfected with the ASPP2 plasmid (ASPP2 Pl) with or without co-transfection with CHOP siRNA (CHOP si). (a and c) Immunoblot assay was used to detect the levels of DRAM, CHOP and ASPP2 in Hep1-6 (a) and Huh7 cells (c). (b and d) Immunofluorescence assay was used to detect cells positive for DRAM expression in Hep1-6 (b) and Huh7 cells (d). Data are shown as the mean±S.E.M. of three independent experiments. (e) Hep1-6 and Huh7 cells were transfected with CHOP si, and then immunoblot was used to detect the expression of DRAM and CHOP. (f) Diagram summarizes our data (details are provided in the Results section)