Figure 5.

PHD2 modulates the expression of HIF target genes. (a–c): U87MG cells were transfected with water (WT), non-specific siRNA (neg.Control) or siRNA against PHD2 (PHD2 kd). Forty-eight hours after transfection, cells were incubated for 24 h in hypoxia (3% O₂). Total mRNA was analyzed for GLUT1, VEGF-A, BNIP3 and ribosomal protein L28 expression by qRT-PCR. Normalized GLUT1/L28 (a) VEGF-A/L28 (b) BNIP3/L28 (c) ratios are shown. The ratios were normalized to WT. The data are shown as the mean±S.E.M. (n=3). *P<0.05, **P<0.01, ***P<0.001