Figure 1.

Suppression of T-cell proliferation and cytokine production by cMSCs. Splenocytes from Balb/c mice were stimulated with anti-CD3 and anti-CD28 antibodies in the presence or absence of cMSCs. Cells were cultured for 3 days. During the final 16 h culture period, 1 μCi ³H-thymidine was added and T-cell proliferation was determined by thymidine incorporation. Both syngeneic (a) and allogeneic (b) cMSCs significantly inhibited T-cell proliferation in a number-dependent manner. After 48 h of stimulation, IFN-γ and IL-4 levels were measured by ELISA. Both cMSCs inhibited IFN-γ and IL-4 production. Similar results were obtained in three independent experiments. M, cMSCs; S, splenocytes, *P<0.001