Figure 4.

siRNAs targeting Bim impair MT103-induced apoptosis. (a) Bim knockdown induced by specific siRNAs was confirmed by western blot. (b and c) Non-transfected and Bim-specific siRNAs-transfected J774 cells were infected with MT103 (MOI 5 : 1 and 10 : 1) during 72 h. (b) Cells were stained with annexinV and 7-AAD, and analysed by flow cytometry. Data in the graphs from four independent experiments are represented as mean±S.E.M. of the percentage of dead cells. (c) Percentage of GFP-positive cells at 0 and 72 h post infection was determined by flow cytometry. Data are shown as mean±S.E.M. of three independent experiments. Statistical analysis was done using one-way ANOVA and Bonferroni's post hoc test. *P<0.05; **P<0.01; ***P<0.001