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. 2014 Jul 31;5(7):e1358. doi: 10.1038/cddis.2014.316

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Copyright © 2014 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/

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PC-3 cells strongly elevate Bid as a pro-apoptotic response to ALDH1L1. (a) Levels of Bcl-2 family proteins in PC-3 cells transiently expressing ALDH1L1. (b) Bcl-XL prevents Bid accumulation and rescues cells from ALDH1L1-induced cell death (MTT assays; inset, Western blot assays). siRNA silencing of Bid rescues PC-3 cells from ALDH1L1-induced cytotoxicity (c) by preventing apoptosis (d and e). Number of apoptotic cells (e) was calculated from annexin V assays shown in (d); average of two independent experiments is shown. MTT assays (b and c) were performed using six replicates for each time point. Statistically significant differences within each time point (b and c) between ALDH1L1-transfected and ALDH1L1/Bcl-XL or ALDH1L1/Bid siRNA-transfected cells are indicated by (*); P<0.0001 (48 h in b and 72 h and 96 h in c); P<0.05 (24 h in b and 48 h in c)