Figure 7.

Full-length Bid translocates to mitochondria of PC-3 cells and induces cell death. (a) Confocal microscopy shows colocalization of endogenous Bid in mitochondria in response to ALDH1L1. (b) Western blot analysis indicates the accumulation of full-length endogenous Bid in both cytosol and mitochondria. (c) Transiently expressed fusion of full-length Bid with GFP is localized in cytosol and mitochondria (wild-type or caspase-8 non-cleavable T59A mutant tagged with GFP were expressed in these experiments). (d) Caspase-8 does not cleave T59A Bid mutant in contrast to the wild-type (WT) protein. (e) Expression of wild-type (WT) Bid as well as T59A or T59D mutants induces cell death (inset shows levels of respective proteins). In experiments described in (d) and (e) non-tagged Bid proteins were expressed. Difference for live-cell count at 72 h between control and Bid expressing cells was highly statistically significant for all three proteins (*P <0.0001). Numbers in (a) and (c) indicate Pearson's correlation coefficient