Figure 3.

RNA and protein levels of globin genes are dissociated in rps19 mutants. (a) mRNA expression of globin gene hemoglobin beta e1 (hbbe1) was not affected or slightly decreased in rps19 mutant embryos at 3 dpf by real-time PCR analysis. (b) Real-time PCR results showed ∼70-fold more hbbe3 mRNA was accumulated in rps19 mutant embryos than control embryos. (c) Western blot results indicated protein levels of both hbbe1 and hbbe3 were dramatically decreased in rps19 mutant embryos at 3 dpf. Histograms represented quantification of western blots. (d) Decreased mpx mRNA was detected in rps19 mutants compared with control embryos. (e) Equal to more mpx protein was produced in rps19 mutants than control embryos at 3 dpf. Histograms represented quantification of western blots. Actin acts as a loading control. The results are representative for three independent experiments. *P<0.05; ***P<0.001; Student's t-test