Figure 6.

Protein production in erythroid cells is also decreased in rpl11 mutants. (a) Real-time PCR results showed more than twofolds of mCherry RNA accumulated in mCherry-positive rpl11 mutant embryos compared with control groups at 3 dpf. (b) Representative images of mCherry-positive embryos of different transgenic lines at 3 dpf. Less mCherry expression was observed in rpl11 mutants than control embryos. Lateral view shown with anterior to the left. (c) Quantification of intensity of mCherry fluorescence in the embryos shown in b. Intensity of mCherry fluorescence shown in rpl11 mutant mCherry control transgenic embryos was ∼50% of the siblings. Approximately, 40% of mCherry fluorescence was exhibited by rpl11 mutant hbbe3 3′UTR mCherry transgenic embryos, compared with the control embryos. The results are representative for three independent experiments. **P<0.01; *P<0.05; Student's t-test