Figure 1.

Histone Modifications at FLC during Vernalization

(A, C, E, and G) H3K27me3 (A), H3K36me3 (C), H3K4me3 (E), and H3K4me2 (G) profiles across the FLC locus for nonvernalized plants (NV) and after different lengths (2, 4, 6, and 8 weeks) of cold treatment, without postcold growth (T0). FLC gene structure is shown schematically at the bottom of (A).

(B, D, F, and H) H3K27me3 (B), H3K36me3 (D), H3K4me3 (F), and H3K4me2 (H) profiles across FLC locus for nonvernalized plants and after different lengths (2, 4, 6, and 8 weeks) of cold treatment, with 7 days postcold growth (T7). Data were presented as the ratio of (FLC/H3) to (reference gene/H3). STM was used as the reference gene for H3K27me3 and ACTIN was used for H3K36me3, H3K4me3 and H3K4me2.

Values represent the average and SEM of three independent biological replicates in all cases. See also Figure S1 and Table S1.