Table II.

Results of property-based statistical analysis and substitution list for Vκ1 family.

Region	Position (IMGT)	Property★	Germline residue	Substitutions
				AL-PCD (n = 91)	Other PCD (n = 53)
FR1	3	FX, HY, SA	Q/W	R, E(3), V(7)	R(2)
	17	pI	D	A, N, G(2)	–
	18	BS, FX	R	K, G(2), N(2), T(2), S(4)	Y, T(3), V(5)
	24	SA, VM	R/Q/W	R, L(2), H(2)	R(3)
	26	FX	S	T, N(4)	R(2)
CDR1	30	BS	S	K, V, T, I, H(2), A(2), D(3), R(4), T(5), G(8), N(18)	V, K, S, R(2), T(2), L(3), N(3), G(5), I(5), A(5)
	31	HY, FX, VM	S/N	E, A, R(2), Y(2), I(4), K(5), N(6), D(6), T(12)	R, Y, H, S(2), K(3), N(3), D(4), T(4), G(5)
FR2	42	FX, HY, SA	Y	W, L(3), F(4)	H, F(2)
	47	pI, SA	G/E	A	–
	52	FX	L/S	I(2), F(3), V(5)	E, A, C, P, F(2), V(5)
CDR2	56	BS, FX	A/D/K	A, N, P, E(2), D(3), S(5), G(11)	V, K, N, T, S(2), A(3), G(3), E(4), D(4)
FR3	67	BS	L	F, M, V(2)	–
	68	FX	Q/E	K, L, A, R, E, I, D, Q(5)	A, R, Q(2), E(3), L(3), H(3)
	69	BS	S/T	K, T, N, P, R(2), A(3), I(3), G(4)	K, P, N(2), A(2), S(2), R(2), G(3), I(3)
	72	HY, pI, SA, VM	P	T, A(2), S(6)	T
	75	BS, pI, SA, VM	R	I, T, H, S(2), K(5), N(9)	M
	86	FX, pI	D/E	R, G(2), E(7), H(7), N(14)	N, Y, V(2), D(2), E(2)
	88	VM	T	Y, S(3), I(3), N(6)	A(2), S(4)
	97	VM	D/E	A, G(2), D(5)	V, D, E
	103	pI, VM	Y	S(2), F(3), H(4)	F(2)
CDR3	107	BS, FX, VM	S/Y/A	F, G, T	Y, N, D, E, I, H, R, G(2), T(2), S(3)
	108	BS	Y/D/N	T, R, F, Q, A(2), D(3), K(3), N(3), E(3), H(3), Y(5), S(8)	Y, I, E, Q(2), T(2), S(3), N(3), H(4)
	109	FX	S/N	R, Y, I, H, K(2), F(2), N(4), S(5), D(10), T(14)	N, C, F, G, R(3), T(5), D(6), S(7)

HY, modified Kyte-Doolittle hydrophobicity scale; FX, average flexibility index; VM, average volume of residue; SA, average accessible surface area; pI, isoelectric point; BS, conformational parameter of beta-structure.

^★Property values identified to be significantly different between the AL-PCD and other-PCD groups.