Figure 2.

Aberrant embryonic-uterine orientation and decidual patterning in Rbpj^d/d females. (A) Representative cross-sections of Rbpj^f/f and Rbpj^d/d implantation sites on days 6 and 8. The narrow columns show a magnified embryonic image of the left pictures. The black dashed line shows the uterine long axis, and the yellow dashed line shows the embryonic long axis. AM, antimesometrial pole; M, mesometrial pole. Scale bar, 500 μm. (B) Schematic diagram illustrating the embryonic orientation within the uterus and the relative position of the section plane. The embryonic axis is parallel to the uterine M-AM axis in the Rbpj^f/f uteri, while the embryonic axis (delineated by the red dashed line) is deflected from the uterine axis in the Rbpj^d/d uteri. The angles of deviation are indicated as ∠θ. (C) The percentage of uterine-embryo disorientation on day 8 for Rbpj^f/f and Rbpj^d/d implantation sites. The threshold of ∠θ analyzed is > 10°. (D) The value of ∠θ during post-implantation stages in Rbpj^f/f and Rbpj^d/d implantation sites. n = 80-90 implantation sites for each time point. (E) The ratio of the uterine short (X) and long (Y) axes during days 6-8 in the Rbpj^f/f and Rbpj^d/d implantation sites, n= 80-90 implantation sites for each time point. Data shown represent the mean ± SEM. ^*P < 0.05. (F) Illustration for measurements of embryo length and embryo area (including ectoplacental cone (epc)) in Rbpj^f/f and Rbpj^d/d implantation sites. (G, H) Average embryo length (G) and embryo area (H) in Rbpj^f/f and Rbpj^d/d implantation sites. Data shown represent the mean ± SEM. ^***P < 0.01. (I) In situ hybridization of Cerl1 in day 8 implantation sites and embryos isolated from Rbpj^f/f and Rbpj^d/d mice.