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. 2014 Jul 15;24(8):977–993. doi: 10.1038/cr.2014.94

Figure 5.

Figure 5

Tom70 mediates pathological hypertrophy of cardiomyocytes by regulating intracellular oxidation states. (A) Measurements of superoxide generation in primary cardiomyocytes treated with ET-1 and Tom70 siRNA using a MitoSOX Red probes. Effects of an antioxidant NAC (2 mM) and catalase on cardiomyocyte size (B-C) and the expression levels of ANP, BNP and α-MHC (D-F). (G) MitoSOX Red staining indicates high ROS generation in the Tom70-deficient hearts of zebrafish (white dotted line) compared with MO-ctrl-injected embryos at 2 dpf. The same exposure intensity was used in different experimental groups. (H) qPCR analysis of the indicated genes in zebrafish hearts injected with MO-Tom70ATG for 48 h with or without NAC (2 mM). NS: nonsignificance. *P < 0.05 versus the respective control using one-way ANOVA. #P < 0.05, δP < 0.05.