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. 2014 Jul 15;24(8):977–993. doi: 10.1038/cr.2014.94

Figure 7.

Figure 7

Cardiomyocytes with Tom70 overexpression are resistant to prohypertrophic insults. (A) Representative images of primary cardiomyocytes treated with ISO, AngII and ET-1 in the presence or absence of Tom70 overexpression. (B) Pooled data from A. (C) mRNA expression of the hypertrophic marker ANP. All the data were obtained from three independent experiments. (D) Western blot analysis of Tom70 proteins in mouse hearts at the indicated times following the direct injection of Adv-Tom70 into the ventricular cavity. (E) Analysis of left ventricular posterior wall thickness (LVPWTd) of mice after ISO injection for 7 days. Top, typical echocardiographic images. Bottom, representative H&E staining. (F) Protein expression analysis of the cardiac hypertrophy marker ANP in mice treated with ISO for 7 days. GAPDH expression was used as a loading control. (G) Superoxide generation of mouse hearts with or without Tom70 overexpression was analyzed 7 days after ISO infusion. n = 12 per ISO treatment group, n = 11 per ISO treatment with Tom70 overexpression group, n = 8 per vector injection group, n = 7 per control group. *P < 0.05 versus the respective control using one-way ANOVA. #P < 0.05.