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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Hum Mutat. 2013 Sep 4;34(11):1467–1471. doi: 10.1002/humu.22395

Figure 2.

Figure 2

Functional analysis of the deletion. A: Splicing pattern of FOXF1 minigenes, cloned into pcDNA3, bearing normal and truncated copies of the FOXF1 intron. Boxes 1 and 2 correspond to intron-flanking regions of the FOXF1 exon 1 and 2, respectively. The 0.4 kb band represents RT-PCR product of correctly spliced mRNA of minigenes from plasmids transfected into human fetal lung fibroblasts. PCR primers were partially homologous to the MCS of the pcDNA3 vector to prevent amplification of cDNA from the FOXF1 endogenous transcript. B: Regulation of the FOXF1 promoter activity by different intronic regions cloned upstream of the FOXF1 promoter in pGL4.10 vector in fetal lung fibroblasts. The boxes in the plot cover percentiles 25-75 and the bar within each box represents the median. Mann-Whitney P values are shown for indicated comparisons next to the box-plot.