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. Author manuscript; available in PMC: 2014 Aug 6.
Published in final edited form as: Biochem Pharmacol. 2013 Feb 14;85(9):1398–1404. doi: 10.1016/j.bcp.2013.02.010

Fig. 5.

Fig. 5

Effect of SFN on the binding of Nrf2 promoter region to acetylated histone 3 (Ac-H3). ChIP assay was performed as described in Material & Methods using antibody against Ac-H3. Regular PCR (A) and qPCR (B) were performed to analyze the enrichment of DNA binding to Ac-H3 using primers which amplify the DNA sequence containing the first five CpGs in the promoter region of Nrf2 gene. (A) Regular PCR was performed to compare the immunoprecipitated DNA versus their inputs of each treatment, and a representative result is shown from three independent experiments. (B) The enrichment of the Ac-H3 binding DNA was determined by qPCR based on the standard curve which was obtained from a serial dilution of the inputs. Relative ratio was calculated by the fold change compared to control group. Data are expressed as mean ± SD from three independent experiments. * Different from control, P < 0.05.