. 2014 Aug 7;20(29):10071–10081. doi: 10.3748/wjg.v20.i29.10071

Table 2.

Methylation-specific polymerase chain reaction primers and real-time polymerase chain reaction primers for KISS1

Primer name	Sequence	Annealing temp (°C)	Product (bp)
KISS1 MSP primer	5’-AAAGTTTCGTTTCGGAGGGTTC-3’	58	172
KISS1 MSP primer	5’-CTTTTATAAAACCCGAAATAACG-3’	58	172
KISS1 un-MSP primers	5’-AAAGTTTTGTTTTGGAGGGTTT-3’	58	173
KISS1 un-MSP primers	5’-AAAGTTTTGTTTTGGAGGGTTT-3’	58	173
KISS1 real-time PCR primers	5’-ACCTGGCTCTTCTCACCAAG-3’	60	201
KISS1 real-time PCR primers	5’-TAGCAGCTGGCTTCCTCTC-3’	60	201
β-actin primers	5’-ATCTGGCACCACACCTTCTACAATGAGCTGCG-3’	60	837
β-actin primers	5’-CGTCATACTCCTGCTTGCTGATCCACATCTGC-3’	60	837

PCR: Polymerase chain reaction.