Table 1.

Reporting standards for in vitro experiments

Parameter	Notes
Mouse strain	How was the bone marrow isolated and processed?
Starting cell number, media and supplements	Media (DMEM versus RPMI) has substantial effects of growth rate, development and activation status
Tissue culture conditions	Different types of plastic affect macrophage growth and activation. Tissue culture conditions should be documented for reproducibility
Time of culture	What were the precise conditions used? Were cytokines and/or media supplemented during the culture period
Source and concentration of differentiation cytokines	The source and concentration of CSF-1
Macrophage yield	The yield relative to the starting number should be recorded
Activation conditions	Variables include whether the macrophages were rested prior to activation and how, whether CSF-1 was present in the activation cultures, the source and concentrations of the activating agents, and the time to assay
Processing and analysis	How were the cells processed, and what marker readouts used