Table 4.
qPCR-derived ISG gene expression data for 293T cells transfected with an IRF-1 expression vector
| 1 μg IRF-1 | 5 μg IRF-1 | 10 μg IRF-1 | |
|---|---|---|---|
| IRF-1 | 6.4 × 105 | 6.4 × 107 | 1.0 × 108 |
| OAS1 | 2.7 | 32 | 49 |
| MXA | 11 | 12 | 23 |
| ISG15 | 3781 | 6152 | 1.2 × 104 |
| STAT3 | — | 3.8 | 7.6 |
| DDX48 | 4.5 | 29 | 57 |
| IFIH1 | 25 | 56 | 111 |
| RSAD2 | 2.7 | 27 | 149 |
| IRF-2 | 5.3 | 10 | 53 |
| IRF-4 | 5.1 | 276 | 377 |
| IRF-7 | 38 | 95 | 118 |
| IFIT1 | 6.2 | 21 | 115 |
| IFIT2 | 42 | 1506 | 2572 |
| IFIT3 | 33 | 620 | 709 |
| IFIT5 | 5.5 | 3.2 | 9.8 |
| IFITM1 | 12 | 462 | 468 |
| IFITM3 | 2.4 | 15 | 22 |
| IFI35 | 8.0 | 143 | 105 |
| IFN-α* | — | — | — |
| IFN-β* | — | — | — |
| IFN-γ* | — | — | — |
| IFITM2* | — | — | — |
| IFITM5* | — | — | — |
| IRF-8* | — | — | — |
293T cells were either untreated or transfected with 1, 5 and 10 μg of the IRF1 expression vector CMVBL-IRF1-HNK or empty vector or treated with PEI transfection reagent only. ISG expression was determined by qPCR 48 hours after treatment. The values shown represent fold change in gene expression compared with untransfected cells. Cells treated with PEI or transfected with 1, 5 and 10 μg of empty vector had a fold change < 2.0.
The — indicates a fold change < 2.0.
*
Genes not up-regulated in their expression in response to IRF1 transfection.