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. Author manuscript; available in PMC: 2014 Aug 6.
Published in final edited form as: Circ Res. 2007 Jun 21;101(3):277–285. doi: 10.1161/CIRCRESAHA.107.155630

Figure 5.

Figure 5

Expression of Nmyc is downregulated by myocardial depletion of Smad4. A through D, Section in situ hybridization analysis was performed on saggital sections of control (A and C) and mutant (B and D) embryos at E10.5 or at E11.5 using an Nmyc antisense probe (purple). Samples were counterstained with nuclear fast red (red). E, Proteins were extracted from control and mutant embryonic hearts at E11.5, and Western blot analysis was performed with an anti-Nmyc antibody. β-Actin was used as a loading control.