Figure 3.

Effect of Cp-BF on the transcriptional regulation of inflammatory genes. (a) The mRNA levels of iNOS, IL-6, and TNF-α were determined by semiquantitative RT-PCR. (b) HEK293 cells cotransfected with plasmid constructs NF-κB-Luc, CREB-Luc, or AP-1-Luc (each 1 μg/mL), and β-gal (as a transfection control) were treated with Cp-BF in the presence or absence of PMA (100 nM; for NF-κB and AP-1-Luc) or forskolin (100 nM for CREB-Luc). Luciferase activity was measured by a luminometer. (c) Levels of nuclear NF-κB (p65 and p50) or AP-1/c-Jun were determined by immunoblot analysis of the nuclear fractions of LPS-treated RAW264.7 cells. *P < 0.05 and **P < 0.01 compared to control.